Thanks for your answer. I put this question, because i made a paternity teste with my two childs, they are twins (not identical) and their mother. One of them give 99,9999% (child 1) and the other 99,99999% (child 2)so i was curious to know why was the diference. Even because the one(child 1) wich get 99,9999% is the one that is more identical to me. Both of them had that scenario in Penta D (mother has in a locci (Penta D) the alleles 9-10 and the child has in the same locci (Penta D) 9-10) but only child 1 had same scenario in D5S818, that’s why the probability of paternity was lower correct?
I had a match with both childs at all 15 locations. So even with this scenario i have no reasons to assume that there is anyone else (even related) that can be the father of them, because according to your experience only the true father will macth all 15 markers, even with this scenario.
Thanks in advance.
Best regards.

Sorry it took me a bit to respond to your question, but I wanted to get some feed back from our Lab Director. This is what he has to say:

“This is a very good question and the answer is quite simply, we do not know which Allele was given by mother but we can take this uncertainty into account in our statistical calculations. To explain, we need take a step back and understand how we account for such events such that we can arrive at a Probability of Paternity. To begin, each DNA marker that is used in a paternity test is given a Paternity Index (PI) which is a calculated value based upon the inheritance scenario and genotypes that exist. The PI reflects the weight of the statistical strength of genetic association given the discovered Mother, Child or Alleged Father genotypes. Broken down, the PI is simply a ratio of the test of two different hypotheses: Numerator (the probability that the Alleged Father of a certain race is the Child’s true biological father given the genetic evidence seen) vs. Denominator (probability that a random man of a particular race could be the biological father of the child given the genetic evidence seen). PI must be calculated for each genetic marker (CSF1PO, Penta D, Penta E, FGA, etc.). All PI’s are then multiplied together to get a Combined Paternity Index (CPI). The CPI is then converted to a probability of paternity (such as 99.99%). These are the highest-level of calculation steps that are performed.”

I added a page to the site that walks though your scenario in in great detail. You can view it here:
Understanding Paternity Index

IDENTIGENE’s paternity test is performed in an accredited paternity testing lab. You can see IDENTIGENE’s lab accreditation here. The accreditation process means that IDENTIGENE’s paternity testing lab undergoes yearly reviews of it’s process for accuracy and professionalism.

The “peace of mind” paternity test, the one available from your local drug store, is a good test if you are looking for personal information only. IDENTIGENE also does legal purpose paternity tests, with chain of custody, that can be used if you need to use your paternity test results in court.

You are right on, the swab should be rubbed inside the cheek, turning the swab to coat all sides. Firmly rubbing the swab for 30 seconds should be sufficient. You can read more information on using the the IDENTIGENE paternity test kit here, or feel free to call any of our test consultants with questions (1-888-404-4363).

I was wondering how legit this pregnancy test is? Are professional lab techs perform this test? Is this the right option to choose if curious? And should the cotton swab turn all around in the inner cheek? For how long?

I’m so sorry that this is so stressful for you right now, and I understand why you would be concerned. However, looks REALLY aren’t a good way to determine paternity. I’m sure you’ve had cases where you’ve met someone or seen someone who looked JUST like a celebrity. It’s the same thing here, you just can’t go by physical features.

Are sure that the correct people’s DNA was submitted and that there was no way that samples could have been switched before arriving at the lab? You didn’t say who did the testing, but did you use an AABB accredited laboratory? If both of those are true, with the same result on two tests, I think your result is probably accurate.

The only other thing I would recommend that you could do, is do a legal test. The test is the same, but with the legal test, the identity of all the participants is confirmed, and the samples handled by an independent third party to be sure they are not falsified or tampered with in any way. A Legal Paternity test is more expensive than a home test, however.